Simplified radioimmunoassay for diagnostic serology.
- 1 November 1972
- journal article
- Vol. 24 (5), 742-9
Abstract
A simplified, indirect radioimmunoassay is described for Escherichia coli, vaccinia virus, and herpesvirus. The antigens were affixed to glass cover slips; thus both the primary and secondary reactions take place on the cover slips, and the unbound antiserum is easily separated from the bound antiserum by rinsing. Rabbit or human immune sera were reacted with the antigens, and the primary immune complex was quantitated by a secondary reaction with (125)I-indicator globulin (anti-rabbit or anti-human). A direct relationship between the antiserum concentration and the (125)I absorption was established. Variations in titers were detectable, and the titers were comparable to complement fixation titers. Homologous and heterologous reactions were distinguishable. The method affords an objective, quantitative, and qualitative evaluation of antibody, and results are reproducible.This publication has 11 references indexed in Scilit:
- AN APPROACH TOWARD AUTOMATION OF ANTINUCLEAR ANTIBODY (ANA) DETERMINATIONAnnals of the New York Academy of Sciences, 1971
- AUTOMATION OF AN INDIRECT FLUORESCENT ANTIBODY TEST FOR SYPHILISAnnals of the New York Academy of Sciences, 1971
- Frequency of Antibody to Hepatitis-Associated Antigen As Measured by a New Radioimmunoassay TechniqueThe Journal of Immunology, 1971
- Application of the Paired Radioiodine-Labeled Antibody Technique (Prilat) to the Detection of Adenovirus 12 Tumor (T) AntigenThe Journal of Immunology, 1970
- General principles of radioimmunoassayClinica Chimica Acta; International Journal of Clinical Chemistry, 1968
- Application of the Paired Label Radioantibody Technique to Tissue Sections and Cell SmearsThe Journal of Immunology, 1967
- A Method of Trace Iodination of Proteins for Immunologic StudiesInternational Archives of Allergy and Immunology, 1966
- THE PREPARATION OF 131I-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITYBiochemical Journal, 1963
- Precipitation of Radiolabeled Poliovirus with Specific Antibody and AntiglobulinThe Journal of Immunology, 1962
- The serum proteins in multiple myelomatosisBiochemical Journal, 1940