Ribosome structure: localization of 3' end of RNA in small subunit by immunoelectronmicroscopy.
- 1 August 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (8), 3769-3773
- https://doi.org/10.1073/pnas.76.8.3769
Abstract
The 3'' end of the RNA in the 30S ribosomal subunit of Escherichia coli was modified by oxidation with sodium periodate and conjugation with the (mono) dinitrophenyl derivative of ethylenediamine. Antibodies, induced with dinitrophenyl-bovine serum albumin, interact with the modified ribosomal subunits. Electron micrographs of negatively stained antibody-subunit complexes show individual ribosomal subunits to which a single antibody molecule is bound and subunit dimers cross-linked by an Ig[immunoglobulin]G molecule. The modified 3'' terminus was localized to a single site on the upper portion of the platform region of the 30S subunit. This location is consistent with earlier placements of proteins that react with the 3'' end of the RNA.This publication has 31 references indexed in Scilit:
- Fluorescence studies of the accessibility of the 3′ ends of the ribosomal RNAs in Escherichia coli ribosomes and subunitsNucleic Acids Research, 1979
- Ribosomal proteins localized at a single region of the large subunit by immune electron microscopyJournal of Molecular Biology, 1978
- Localization of the site of adenylylation of glutamine synthetase by electron microscopy of an enzyme-antibody complexProceedings of the National Academy of Sciences, 1978
- The sequence of Escherichia coli ribosomal 16 S RNA determined by new rapid gel methodsFEBS Letters, 1978
- Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.Proceedings of the National Academy of Sciences, 1978
- Ribosome StructureAnnual Review of Biochemistry, 1978
- Structure and Function of the Bacterial RibosomeAnnual Review of Biochemistry, 1977
- Ribosome structure: localization of N6,N6-dimethyladenosine by electron microscopy of a ribosome-antibody complex.Proceedings of the National Academy of Sciences, 1977
- TERMINAL-SEQUENCE STUDIES OF HIGH-MOLECULAR-WEIGHT RIBONUCLEIC. THE REACTION OF PERIODATE-OXIDIZED RIBONUCLEOSIDES, 5′-RIBONUCLEOTIDES AND RIBONUCLEIC ACID WITH ISONIAZIDBiochemical Journal, 1965
- A method for the determination of nucleotide sequence in polyribonucleotidesBiochemical Journal, 1954