An avidin‐biotinyl‐propranolol complex for β‐adrenergic receptor characterization
- 1 January 1978
- journal article
- research article
- Published by Wiley in Journal of Supramolecular Structure
- Vol. 9 (2), 243-252
- https://doi.org/10.1002/jss.400090209
Abstract
The synthesis of biotinyl-hexaglycyl-NEDA (abbreviation:BGN), a biotinyl derivative of propranolol, is described. This bifunctional molecule binds with high affinity to the biotin-binding protein, avidin. The duck erythrocyte was used as a model β-receptor system. Formation of an avidin-BGN-β-receptor complex was demonstrated in intact erythrocytes, in erythrocyte ghosts, and in the digitonin-solubilized β-receptor. The avidin-BGN complex will be used for localization and purification of the β-receptor.Keywords
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