Abstract
Broilers were fed 15N labelled wheat and the concentration of the labelled isotope in muscular tissue was determined. A negative correlation was found to exist between the percentage proportion of N in the muscle fractions and the level of 15N frequency established 12 hrs after administration of the labelled ration. Higher rates of tracer incorporation were observed in the muscles of legs and other muscles (predominatly red muscles) than in pectoral muscles (white muscles). The heart was shown to exhibit the lowest N content and the highest level of 15N frequency of all muscle fractions. In view of the fact that some specific difficulties will arise when certain factors (trace elements, vitamins, ergotropic substances, genetic factors) which influence the N metabolism of fattening poultry, are estimated on the basis of the conventional N balance trials the present tracer technique is recommended for use as an alternative method in studies of the kind just described. In the present trial 20% of the wheat N were utilized for the production of meat N in muscular tissue.