INTERMEDIATES OF VITAMIN B1 AND THE GROWTH OF TORULA

Abstract

Eight spp. of Torula were grown in a medium of mineral salts, asparagine and dextrose and the same medium to which the thiazole alone, the pyrimidine alone, or both intermediates of vitamin B1 were added. T. cremoris and T. kefyr failed to grow in any of the 4 media; the growth of T. hansen and T. sphaerica was unaffected by the supplements ; T. laurentii and T. fermentati grew better when both intermediates were used as supplements but were unaffected by either intermediate alone; the growth of T. rosea and T. sanguinea was much increased by the addition of pyrimidine or of pyrimidine and thiazole but unaffected by thiazole alone. By cultivating Phycomyces blakesleeanus in the solns. in which the Torulae had grown the synthesis of both intermediates (or vitamin B1) and of pyrimidine by certain of the Torulae was demonstrated.