Heme Requirement for Reproduction of a Free-Living Nematode
- 3 April 1970
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 168 (3927), 143-144
- https://doi.org/10.1126/science.168.3927.143
Abstract
The free-living hermaphroditic nematode Caernorhabditis briggsae has a nutritional requirement for heme. The organism can be subcultured repeatedly in a chemically defined axenic medium that contains autoclaved bacterial cells (Escherichia coli) and sterols if a hemeprotein-containing fraction from liver is present. Pure myoglobin, hemoglobin, cytochrome c, and hemin, respectively, can substitute effectively for the liver fraction.Keywords
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