Cultivation of Poliomyelitis Virus in Tissue Culture III. Synthetic Medium in Roller Tube Cultures.

Abstract

The Brunhilde, Mahoney, Canadian Eskimo, Lansing, Y-SK, MEF1, Leon, and Saukett strains of poliomyelitis virus have been propagated in roller tube cultures of monkey testicular tissue treated with synthetic Mixture No. 199, devised by Morgan, Morton, and Parker. Virus-induced cytopathogenic changes occurred in the fibroblasts and were similar to those observed in cultures treated with mixtures containing naturally occurring ingredients. Virus and serum titrates in roller tube cultures were read 7 days after infection of the cultures; during this period no change of culture fluid was necessary. The Brunhilde and Lansing strains were propagated in large roller tube cultures treated with Mixture 199, and high titers were obtained. Culture fluids infected with Brunhilde and Lansing viruses from large roller tubes (mixed with adjuvants) were used to immunize groups of monkeys by the intramusc. route, and high serum antibody levels (4.0) developed promptly.