Towards a two‐dimensional database of common human proteins
- 1 January 1992
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 13 (1), 729-732
- https://doi.org/10.1002/elps.11501301156
Abstract
A protein pattern of common human proteins was constructed by comparing the two-dimensional gel electrophoresis (2-DE) protein patterns from five cell lines of different germ layers. Total cell lysate and the isolated and purified nuclei of each cell line were separated by parallel electrophoresis runs in a multiple casting system of highest reproducibility. The computerized image analysis of the digitized 2-DE gels revealed a master protein pattern for each cell line. By comparison of all master protein patterns a 2-DE protein map of only common human proteins was constructed as a basis for a new 2-DE database. In a first step we have started characterizing a number of spots by microsequencing, amino acid composition analysis, and mass spectroscopy.Keywords
This publication has 14 references indexed in Scilit:
- The master two‐dimensional gel database of human AMA cell proteins: Towards linking protein and genome sequence and mapping information (Update 1991)Electrophoresis, 1991
- A strategy for founding a global lymphocyte proteinpaedia and gene catalogueImmunology Today, 1990
- Identification of mouse brain proteins after two‐dimensional electrophoresis and electroblotting by microsequence analysis and amino acid composition analysisElectrophoresis, 1988
- Two‐dimensional electrophoresis. The current state of two‐dimensional electrophoresis with immobilized pH gradientsElectrophoresis, 1988
- Isoelectric focusing in immobilized pH gradients: Principle, methodology and some applicationsJournal of Biochemical and Biophysical Methods, 1982
- Up-dated catalogue of HeLa cell proteins: percentages and characteristics of the major cell polypeptides labeled with a mixture of 16 14C-labeled amino acids.Clinical Chemistry, 1982
- Synthesis of specific identified, phosphorylated, heat shock, and heat stroke proteins through the cell cycle of Saccharomyces cerevisiae.Molecular and Cellular Biology, 1982
- High resolution two-dimensional electrophoresis of human plasma proteins.Proceedings of the National Academy of Sciences, 1977
- Isolation of liver and muscle polyribosomes in high yield after cell disruption by nitrogen cavitationFEBS Letters, 1968
- The phosphatidylinositol kinase of rat brainBiochemical Journal, 1966