A DNA probe to distinguish the species Anopheles quadriannulatus from other species of the Anopheles gambiae complex
- 31 October 1987
- journal article
- Published by Oxford University Press (OUP) in Transactions of the Royal Society of Tropical Medicine and Hygiene
- Vol. 81 (5), 842-846
- https://doi.org/10.1016/0035-9203(87)90048-4
Abstract
DNA probes used previously to distinguish the species Anopheles gambiae sensu stricto, An.arabiensis, An.melas and An.merus were tested against An.quadriannulatus . Using these DNA probes, An.gambiae s.s. and An.quadriannulatus were indistinguishable. A genomic library was constructed for An.quadriannulatus . Differential screening of this genomic library with An.gambiae s.s. and An. quadriannulatus genomic DNAs identified a species-specific, repeated DNA sequence. When used as a hybridization probe, this DNA sequence clearly distinguished An.gambiae s.s. from An.quadriannulatus . A simplified protocol for the use of DNA probes is described which may be used to identify material squashed directly on to nitrocellulose filter paper.Keywords
This publication has 15 references indexed in Scilit:
- DNA probes for species identification of mosquitoes in the Anopheles gambiae complexMedical and Veterinary Entomology, 1987
- Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotinNucleic Acids Research, 1985
- Chromosomal inversion intergradation and incipient speciation inAnopheles gambiaeBolletino di zoologia, 1985
- Rate of turnover of structural variants in the rDNA gene family of Drosophila melanogasterNature, 1982
- Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general methodCell, 1981
- A Biochemical Key to Adult Members of the Anopheles Gambiae Group of Species (Diptera: Culicidae)1Journal of Medical Entomology, 1979
- Enzyme variation in theAnopheles gambiaeGiles group of species (Diptera: Culicidae)Bulletin of Entomological Research, 1978
- Diagnostic allozymes for routine identification of adults of theAnopheles gambiaecomplex (Diptera, Culicidae)Bulletin of Entomological Research, 1976
- Cytological maps for the practical identification of females of the three freshwater species of theAnopheles gambiaecomplexPathogens and Global Health, 1972
- Calcium-dependent bacteriophage DNA infectionJournal of Molecular Biology, 1970