ISOLATION AND IDENTIFICATION OF 3α,17α,21-TRIHYDROXY-5α-PREGNAN-20-ONE (ALLO-TETRAHYDRO-COMPOUND S) AFTER ADMINISTRATION OF 3β, 17α,21-TRIHYDROXYPREGN-5-EN-20-ONE(17α,21-DIHYDROXYPREGNENOLONE)
- 1 August 1963
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 88 (2), 315-318
- https://doi.org/10.1042/bj0880315
Abstract
3[alpha],17[alpha],21-Trihydroxy-5[alpha]-pregnan-20-one (allo-tetrahydro-compound S) has been isolated and identified in human urine after the administration of 3[beta],17[alpha],21-trihydroxypregn-5-en-20one (17[alpha],21-dihydroxypregnenolone) to a normal subject. Its identification was established on the basis of chromatography in four different systems and in 2 systems after acetylation. Its oxidation product was proved to be androsterone by chromatography of the free compound and of the acetate. Of this steroid 60% was found in the leastpolar ester sulfate fraction and 40% in the glucuronide fraction.Keywords
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