Platelet lysate favoursin vitroexpansion of human bone marrow stromal cells for bone and cartilage engineering
- 17 October 2008
- journal article
- research article
- Published by Hindawi Limited in Journal of Tissue Engineering and Regenerative Medicine
- Vol. 2 (8), 472-481
- https://doi.org/10.1002/term.119
Abstract
The heterogeneous population of non-haematopoietic cells residing in the bone marrow (bone marrow stromal cells, BMSCs) and the different fractions and components obtained from platelet-rich plasma provide an invaluable source of autologous cells and growth factors for bone and other connective tissue reconstruction. In this study, we investigated the effect of an allogenic platelet lysate on human BMSCs proliferation and differentiation. Cell proliferation and number of performed cell doublings were enhanced in cultures supplemented with the platelet-derived growth factors (platelet lysate, PL), either with or without the concomitant addition of fetal bovine serum (FBS), compared to cultures performed in the presence of FBS and FGF2. Both in vitro and in vivo osteogenic differentiation were unaltered in cells maintained in medium supplemented with PL and not FBS (Only PL) and in cells maintained in medium containing FBS and FGF2. Interestingly, the in vitro cartilage formation was more effective in the pellet of BMSCs expanded in the Only PL medium. In particular, a chondrogenic differentiation was observed in pellets of some in vitro-expanded BMSCs in the Only PL medium, whereas pellets from parallel cell cultures in medium containing FBS did not respond to the chondrogenic induction. We conclude that the platelet lysate from human source is an effective and even more beneficial substitute for fetal bovine serum to support the in vitro expansion of human BMSCs for subsequent tissue-engineering applications. Copyright © 2008 John Wiley & Sons, Ltd.Keywords
This publication has 48 references indexed in Scilit:
- A tissue engineering approach to bone repair in large animal models and in clinical practiceBiomaterials, 2007
- Accelerated and safe expansion of human mesenchymal stromal cells in animal serum‐free medium for transplantation and regenerative medicineJournal of Cellular Physiology, 2007
- Calcium Sulfate and Platelet-Rich Plasma make a novel osteoinductive biomaterial for bone regenerationJournal of Translational Medicine, 2007
- Simultaneous concentration of platelets and marrow cells: A simple and useful technique to obtain source cells and growth factors for regenerative medicineWound Repair and Regeneration, 2007
- Early effect of platelet‐rich plasma on bone healing in combination with an osteoconductive material in rat cranial defectsClinical Oral Implants Research, 2007
- Tissue‐engineered intervertebral disc and chondrogenesis using human nucleus pulposus regulated through TGF‐β1 in platelet‐rich plasmaJournal of Cellular Physiology, 2006
- Platelet lysates promote mesenchymal stem cell expansion: A safety substitute for animal serum in cell-based therapy applicationsJournal of Cellular Physiology, 2005
- Gene expression profile of human bone marrow stromal cells determined by restriction fragment differential display analysisJournal of Cellular Biochemistry, 2004
- Tissue EngineeringScience, 1993
- Platelet-derived growth factor B chain homodimer enhances chemotaxis and DNA synthesis in normal osteoblast-like cells (MC3T3-E1)Biochemical and Biophysical Research Communications, 1991