Combined Use of Normal and Reversed Phase Thin Layer Chromatography in the Screening for Basic and Quaternary Drugs
- 1 April 1991
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 14 (8), 1435-1446
- https://doi.org/10.1080/01483919108049625
Abstract
The combined use of normal and reversed phase thin layer chromatography in drug screening is evaluated by the mean list length method. A reversed phase system, involving RP-18 plates and aqueous hydrochloric acid - methanol as a mobile phase, is shown to be an effective complementary pair to basic medium-polar normal phase systems. With a set of 140 basic and quaternary drugs, a mean list length of 1.8 is obtained for a TLC/RPTLC pair. The combination is also applicable to hydrophilic drugs extracted as bis(2-ethyl-hexyl)phosphate ion-pairs.Keywords
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