LACK OF T‐CELL MEDIATED CYTOTOXICITY IN M‐MSV SYSTEM DEPENDING ON H‐2 HAPLOTYPES

Abstract

The H-2 restriction phenomenon was evaluated with regard to the immune response to Moloney sarcoma virus (M-MSV)-induced tumors in the mouse. Using an in vitro 51Cr release assay the lytic activity of lymphocytes from M-MSV immune strains of mice, including several H-2 recombinant strains, was determined on leukemic cell lines originally induced in mice of different strains by neonatal infection with Moloney leukemia virus. In analogy with other experimental models, compatibility between effector and target cells at the K and/or D regions was generally necessary and sufficient to obtain the cytotoxic effect. For H-2d and H-2b haplotypes, identity at the K or D region respectively was not sufficient for lysis to occur. Attempts to demonstrate that this lack of activity might be associated with absence of H-2-linked Ir responder genes, were not successful.