Nucleotide sequence and structural analysis of two satellite RNAs associated with chicory yellow mottle virus
- 1 September 1990
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 71 (9), 1897-1903
- https://doi.org/10.1099/0022-1317-71-9-1897
Abstract
The two satellite RNAs associated with CYMV infections were sequenced. The larger (sCYMV-L1) has only linear molecules 1145 nucleotides long, a poly(A) tail, a long open reading frame (ORF) coding for a protein of M r 39636 resembling in composition those of other large nepovirus satellite RNAs, a 5′ leader sequence of 16 nucleotides and a 3′ non-coding region of 40 nucleotides. In vitro translation of sCYMV-L1 yielded a protein product with a size that corresponded to that predicted from the sequence. The smaller satellite (sCYMV-S1) is 457 nucleotides long, has no ORF of significant length and no in vitro messenger activity. Both linear and circular forms of this satellite RNA were detected in infected tissues. Comparison of the sCYMV-S1 primary structure with the sequences of other small nepoviral satellites reveals large regions of homology. Analysis of the secondary structures derived from the sequences of the plus and minus strands suggests possible consensus sequences for their self-cleavage.This publication has 20 references indexed in Scilit:
- A two-dimensional electrophoretic technique for the detection of circular viroids and virusoidsAnalytical Biochemistry, 1983
- A simple and very efficient method for generating cDNA librariesGene, 1983
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- RNA ligation via 2'-phosphomonoester, 3'5'-phosphodiester linkage: requirement of 2',3'-cyclic phosphate termini and involvement of a 5'-hydroxyl polynucleotide kinase.Proceedings of the National Academy of Sciences, 1982
- A rapid boiling method for the preparation of bacterial plasmidsAnalytical Biochemistry, 1981
- Prediction of protein antigenic determinants from amino acid sequences.Proceedings of the National Academy of Sciences, 1981
- The sequence 5′-AAUAAA-3′ forms part of the recognition site for polyadenylation of late SV40 mRNAsCell, 1981
- Structure mapping of 5'-32P-labeled RNA with S1 nucleaseBiochemistry, 1978
- 3'-terminal nucleotide sequence of encephalomyocarditis virus RNA determined by reverse transcriptase and chain-terminating inhibitors.Proceedings of the National Academy of Sciences, 1978
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977