Solid‐Phase Immunoassays for Quantitation of Antibody to Bovine White Matter Proteolipid Apoprotein
- 1 February 1982
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 38 (2), 348-355
- https://doi.org/10.1111/j.1471-4159.1982.tb08635.x
Abstract
Two solid-phase immunoassays have been developed for quantitation of antibodies to bovine white matter proteolipid apoprotein. Conditions were established for optimal specific antibody binding. Water-soluble proteolipid apoprotein was bound to microtiter plates and plates were incubated with test serum. Goat anti-rabbit IgG conjugated with horeseradish peroxidase was used as the second antibody for an enzyme-linked immunospecific assay and 125I-labeled protein A for a radioimmunoassay. Both procedures have been used to follow the time course of anti-proteolipid antibody production in rabbits and to compare different immunization protocols.Keywords
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