INHIBITION OF THE ENZYMIC OXIDATION OF DPNH BY STER HORMONES

Abstract
Low concentrations of a large number of steroid hormones and diethylstilbestrol inhibited reduced diphosphopyridine nucleotide (DPNH) oxidation by enzyme preparations from both mammalian and microbial sources. Neither reduced triphosphopyridine nucleotide (TPNH) or succinate were similarly affected, however. In skeletal muscle, the site of steroid inhibition proved to be the DPNH-cytochrome c reductase reaction. a-Tocopherol and other compounds could competitively reverse the steroid effect.