A Modified Enzymatic Technique for Production of Cell Suspensions of a Murine Fibrosarcoma2, 3

Abstract

Collagenase, elastase, and mild mechanical procedures were used to obtain numerous viable single cells in suspension from a murine fibrosarcoma of C57BL/6 origin. This technique produced suspensions with minimal debris. The suspensions were more than 90% viable and yielded over 1.25×10⁸ cells/g of solid tumor.