Matrix-Implanted Laser Desorption/Ionization Mass Spectrometry
- 6 November 2004
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 76 (24), 7288-7293
- https://doi.org/10.1021/ac049123i
Abstract
The implantation of low-velocity massive gold clusters is shown to be a method of choice for homogeneous incorporation of a metallic matrix into the near-surface region of a solid biopolymer for subsequent laser desorption/ionization (LDI) MS analysis. Matrix implanted (MI)LDI spectra from cluster-implanted pure test peptide or tissue exhibit molecular ion peaks similar to those observed by matrix-assisted LDI. Moreover, the ion emission is very reproducible from any spot on the surface of these test samples. MILDI promises to be a powerful technique for mass spectrometric analysis of native biological samples as demonstrated by the first results on rat brain tissues.Keywords
This publication has 8 references indexed in Scilit:
- Orthogonal time-of-flight secondary ion mass spectrometric analysis of peptides using large gold clusters as primary ionsRapid Communications in Mass Spectrometry, 2004
- Effect of Local Matrix Crystal Variations in Matrix-Assisted Ionization Techniques for Mass SpectrometryAnalytical Chemistry, 2003
- Heterogeneity within MALDI Samples As Revealed by Mass Spectrometric ImagingAnalytical Chemistry, 1999
- Laser Desorption/Ionization Mass Spectrometry of Peptides and Proteins with Particle Suspension MatrixesAnalytical Chemistry, 1998
- Graphite/Liquid Mixed Matrices for Laser Desorption/Ionization Mass SpectrometryAnalytical Chemistry, 1996
- Graphite surface-assisted laser desorption/ionization time-of-flight mass spectrometry of peptides and proteins from liquid solutionsAnalytical Chemistry, 1995
- Impact of slow gold clusters on various solids: nonlinear effects in secondary ion emissionNuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 1991
- Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltonsAnalytical Chemistry, 1988