Über die direkte Wirkung von Röntgenstrahlen auf Proteine, Peptide und Aminosäuren

Abstract

The primary effect of X-irradiation (2-30 million r) on crystalline lysozyme was investigated by physico-chemical (electro-phoresis, ultracentrifugation), chemical, biochemical and biological methods. A series of closely related, although less basic proteins of different mol. wt. were formed the amino acid constituents of which changed in part into other compounts as a result of irradiation. In amino-and carboxyl-terminal amino acids of the irradiated proteins differences, compared with lysozyme, were found only in the carboxyl terminal groups. The biological activity of the proteins remained practically unchanged even after irradiation with a dose of 5 million r.