Enrichment of Antibody Plaque-Forming Cells of Spleen by Sedimentation at Unit Gravity

Abstract

Spleen cells from mice immunized with sheep erythrocytes were sedimented at unit gravity in a sucrose gradient. Cells forming hemolytic antibody plaques sedimented more rapidly than the bulk of the nonplaque-forming cells, resulting in a partial separation with enrichments of up to 18-fold over their starting concentration.