RAPD typing of clinical isolates of Staphylococcus haemolyticus
Open Access
- 1 February 1994
- journal article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 18 (2), 86-89
- https://doi.org/10.1111/j.1472-765x.1994.tb00811.x
Abstract
The randomly amplified polymorphic DNA (RAPD) assay was used to generate DNA fingerprints from clinical isolates of Staphylococcus haemolyticus isolated from patients treated with continuous ambulatory peritoneal dialysis and previously subjected to a combination of typing methods. The RAPD profiles generated with one of six randomly designed 10-mer primers allowed visual discrimination of strains. Good correlation with the original typing scheme was achieved but RAPD typing allowed discrimination of strains previously indistinguishable.Keywords
This publication has 11 references indexed in Scilit:
- Genomic fingerprinting of "Haemophilus somnus" isolates by using a random-amplified polymorphic DNA assayJournal of Clinical Microbiology, 1993
- Evaluation of methods for typing coagulase-negative staphylococciJournal of Medical Microbiology, 1992
- RAPD analysis ofCampylobacterisolates: DNA fingerprinting without the need to purify DNALetters in Applied Microbiology, 1992
- Rapid genomic fingerprinting of Lactococcus lactis strains by arbitrarily primed polymerase chain reaction with 32P and fluorescent labelsApplied and Environmental Microbiology, 1992
- Optimization of the PCR program for RAPD analysisNucleic Acids Research, 1992
- DNA polymorphisms amplified by arbitrary primers are useful as genetic markersNucleic Acids Research, 1990
- Fingerprinting genomes using PCR with arbitrary primersNucleic Acids Research, 1990