Influence of glucose on Ehrlich cell volume, ion transport, and membrane potential
- 1 May 1982
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Cell Physiology
- Vol. 242 (5), C326-C332
- https://doi.org/10.1152/ajpcell.1982.242.5.c326
Abstract
Incubating Ehrlich ascites tumor cells with 10 mM glucose at room temperature resulted in the following changes. The cells shrank, reaching a minimum volume after 1 h. The decrease in cell volume was 50–90% inhibited by 1 mM furosemide. The mmol K+ and Cl-/mg dry wt decreased, and mmol Na+/mg dry wt increased over the 1 h incubation. The net loss of KCl was inhibited by 1 mM furosemide. Immediately after the addition of glucose, the influx of 86Rb sensitive to ouabain decreased, whereas the influx sensitive to furosemide increased. The total influx of 86Rb with glucose was similar to that of controls. The effluxes of 86Rb and 36Cl increased immediately after the addition of glucose. These effluxes did not increase, however, in the presence of 1 mM furosemide. Initially the ouabain-sensitive Na+ efflux was not changed with glucose, but the ouabain-insensitive Na+ efflux decreased. Furosemide (1 mM) did not influence Na+ efflux. With time the ouabain-sensitive Na+ efflux increased as cellular Na+ levels rose so that at 1 h the ouabain-sensitive Na+ efflux from glucose-treated cells was 2.5–3 times that of control cells. The potential difference across the membrane gradually became more negative by apprdoximately 25 mV, reaching a maximum after 1 h. The hyperpolarization was reversed by 1 mM ouabain. The changes in ionic fluxes on the addition of glucose are compared with changes in ionic fluxes seen during volume regulation.This publication has 19 references indexed in Scilit:
- Osmoregulatory Salt Transporting Mechanisms: Control of Cell Volume in Anisotonic MediaAnnual Review of Physiology, 1981
- K+ influx components in ascites cells: The effects of agents interacting with the (Na++K+)-pumpThe Journal of Membrane Biology, 1980
- The influence of cellular amino acids and the Na+: K+ pump on the membrane potential of the Ehrlich ascites tumor cellBiochimica et Biophysica Acta (BBA) - Biomembranes, 1978
- A reassessment of decreased amino acid accumulation by Ehrlich ascites tumor cells in the presence of metabolic inhibitors.The Journal of general physiology, 1977
- Localization of calcium binding sites associated with the calcium spike in barnacle muscleThe Journal of Membrane Biology, 1976
- Potassium transport in normal and transformed mouse 3T3 cellsJournal of Cellular Physiology, 1976
- Cell cycle dependent changes in potassium transportJournal of Cellular Physiology, 1976
- Cation permeability and ouabain-insensitive cation flux in the Ehrlich ascites tumor cellThe Journal of Membrane Biology, 1975
- Role of ATP on the initial rate of amino acid uptake in ehrlich ascites cellsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1974
- Cation gradients, ATP and amino acid accumulation in Ehrlich ascites cellsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1971