Studies on the Nutritional Requirements of Streptomyces griseus for the Formation of Streptomycin

Abstract

The volume of medium in shake-flask cultures of 5. griseus plays an important role in the concentration of streptomycin obtained in the broth. Beef extract is not required for streptomycin production in a medium containing soybean meal, glucose, and NaCl. In fact, the addition of beef extract to such a medium in shake flasks delays somewhat the production of this antibiotic. It is necessary to add an inorganic salt, e.g., NaCl to soybean meal media for streptomycin production. Preliminary studies indicate that Na2SO4 may be substituted for NaCl, but the substitution of MgCh for either of these 2 electrolytes gives lower streptomycin yields. Beef extract may supply a certain amt. of the salt required in a soybean meal medium.