A cDNA clone encoding part of the major 25000-dalton surface membrane antigen of adultSchistosoma mansoni
- 1 January 1989
- journal article
- research article
- Published by Springer Nature in Zeitschrift Fur Parasitenkunde-Parasitology Research
- Vol. 75 (4), 280-286
- https://doi.org/10.1007/bf00931812
Abstract
Immunoscreening of an adultSchistosoma mansoni cDNA expression library, using antibodies raised against purified adult worm tegumental surface membranes, identified a recombinant clone containing a 141-bp insert. Antibodies raised against the recombinant antigen bound specifically to the tegument of adult worms and immunoprecipitated the major 25000-dalton surface membrane antigen as well as a 22000-dalton nascent polypeptide generated by cell-free translation of adultS. mansoni mRNA. The mature 25000-dalton antigen was found to be precipitated by antibodies from infected mice, rats and humans.Keywords
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