EELS data acquisition, processing and display for the Zeiss CEM 902 based on lotus 1–2‐3®: application examples from a biological system and inorganic transition metal compounds

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Abstract
A personal computer combined with lotus 1‐2‐3® software, including the RS232 module of lotus measure® and a 12‐bit ADC, has been used for data acquisition of electron energy‐loss spectroscopy (EELS) spectra with the Zeiss CEM 902. The internal macro language of lotus 1‐2‐3 allows a menu‐driven procedure. Macro‐programs partly combined with external FORTRAN programs can be chosen from the menu for background subtraction, removal of multiple scattering effects by deconvolution, elemental quantification and several utilities. For special applications or conditions the macro programs can easily be modified. Spectra from crystals of two inorganic transition metal compounds, ruthenium trichloride and vanadium disulphide, and from a biological sample are presented as examples of the application of this software.