Retention of the oxygens at C-2 and C-3 of D-ribulose 1,5-bisphosphate in the reaction catalyzed by ribulose-1,5-bisphosphate carboxylase
- 19 September 1978
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 17 (19), 4041-4044
- https://doi.org/10.1021/bi00612a026
Abstract
Ribulose-1,5-bisphosphate carboxylase [EC 4.1.1.39, from Rhodospirillum rubrum] catalyzes the conversion of D-ribulose 1,5-bisphosphate and CO2 to 3-phospho-D-glycerate, with retention of the O2 atoms at both C-2 and C-3 of the substrate. This observation is consistent with mechanistic pathways involving an enediol intermediate and eliminates suggested mechanisms that involve covalent intermediates between the enzyme and ribulose 1,5-bisphosphate in which the substrate O2 at C-2 or C-3 is compulsorily lost.This publication has 11 references indexed in Scilit:
- Inhibition of ribulose-1,5-bisphosphate carboxylase/oxygenase by xylulose 1,5-bisphosphateJournal of Biological Chemistry, 1977
- Reaction of ribulosebisphosphate carboxylase from Rhodospirillum rubrum with the potential affinity label 3-bromo-1,4-dihydroxy-2-butanone 1,4-bisphosphateBiochemical and Biophysical Research Communications, 1977
- Essential primary amino groups of ribulose bisphosphate carboxylase/oxygenase indicated by reaction with pyridoxal 5′-phosphateArchives of Biochemistry and Biophysics, 1977
- Energetics of triosephosphate isomerase: the nature of the proton transfer between the catalytic base and solvent waterBiochemistry, 1976
- Inhibition of D-ribulose 1,5-bisphosphate carboxylase by pyridoxal 5′-phosphateBiochemical and Biophysical Research Communications, 1976
- Catalysis of an oxygen-exchange reaction of fructose 1,6-diphosphate and fructose 1-phosphate with water by rabbit muscle aldolaseBiochemistry, 1968
- Structure and function of chloroplast proteinsArchives of Biochemistry and Biophysics, 1968
- [10] Assay of inorganic phosphate, total phosphate and phosphatasesPublished by Elsevier ,1966
- DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*Annals of the New York Academy of Sciences, 1964
- The Formation of Isocitratase by the AthiorhodaceaeJournal of General Microbiology, 1960