Conjugation of lectins with fluorochromes: An approach to histochemical double labeling of carbohydrate components
- 1 January 1978
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 56 (3-4), 265-273
- https://doi.org/10.1007/bf00495988
Abstract
Methodical investigations on the coupling of lectins (Con A, LcL, WGA, RcA) to tetramethylrhodamine isothiocyanate (TRITC) are reported. 20 μg of TRITC per mg of lectin were found to be the optimal amount of TRITC for the conjugation. With this fluorochrome: protein ratio conjugates were produced which resulted in a specific and brilliant fluorescence in tissue staining. The optimally conjugated lectins were separated on DEAE-Sephadex-A 50. Using two different lectins which were conjugated with TRITC or FITC, respectively, a double labeling of different lectin-binding sites in tissue sections was achieved.This publication has 43 references indexed in Scilit:
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