Partial Purification of Cattle Serum Transferrin Using Rivanol.

Abstract

Inherited variations in the serum proteins of many animal species is a useful approach to the study of gene-controlled protein specificity. Cur purpose was to prepare cattle transferrin pure enough for chemical studies of the phenotypic differences. A ratio of 3 volumes of 0.4% rivanol (2-ethoxy 6,9-diaminoacridine lactate) to one volume of serum was used to purify cattle transferrin. After treatment, the supernatant fraction contained predominantly transferrin and gamma globulin. No albumin was detectable by starch gel or paper electrophoresis. Fractionation of serum from cattle of phenotypically different transferrin types yielded transferrin with the same pattern and mobility in starch gel electrophoresis as in the original serum.