Molybdenum cofactor transfer from bacteria to nematode mediates sulfite detoxification
- 25 March 2019
- journal article
- research article
- Published by Springer Nature in Nature Chemical Biology
- Vol. 15 (5), 480-488
- https://doi.org/10.1038/s41589-019-0249-y
Abstract
The kingdoms of life share many small molecule cofactors and coenzymes. Molybdenum cofactor (Moco) is synthesized by many archaea, bacteria, and eukaryotes, and is essential for human development. The genome of Caenorhabditis elegans contains all of the Moco biosynthesis genes, and surprisingly these genes are not essential if the animals are fed a bacterial diet that synthesizes Moco. C. elegans lacking both endogenous Moco synthesis and dietary Moco from bacteria arrest development, demonstrating interkingdom Moco transfer. Our screen of Escherichia coli mutants identifies genes necessary for synthesis of bacterial Moco or transfer to C. elegans. Developmental arrest of Moco-deficient C. elegans is caused by loss of sulfite oxidase, a Moco-requiring enzyme, and is suppressed by mutations in either C. elegans cystathionine gamma-lyase or cysteine dioxygenase, blocking toxic sulfite production from cystathionine. Thus, we define the genetic pathways for an interkingdom dialogue focused on sulfur homeostasis.This publication has 45 references indexed in Scilit:
- The million mutation project: A new approach to genetics in Caenorhabditis elegansGenome Research, 2013
- CloudMap: A Cloud-Based Pipeline for Analysis of Mutant Genome SequencesGenetics, 2012
- Dual Role of the Molybdenum Cofactor Biosynthesis Protein MOCS3 in tRNA Thiolation and Molybdenum Cofactor Biosynthesis in HumansJournal of Biological Chemistry, 2012
- Allele-Specific Suppressors of lin-1(R175Opal) Identify Functions of MOC-3 and DPH-3 in tRNA Modification Complexes in Caenorhabditis elegansGenetics, 2010
- Single-copy insertion of transgenes in Caenorhabditis elegansNature Genetics, 2008
- Molybdoproteomes and Evolution of Molybdenum UtilizationJournal of Molecular Biology, 2008
- Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collectionMolecular Systems Biology, 2006
- Investigation of the Early Steps of Molybdopterin Biosynthesis in Escherichia coli through the Use of in Vivo Labeling StudiesJournal of Biological Chemistry, 1995
- Inactivation of thioredoxin by sulfite ionsFEBS Letters, 1990
- Sulphite toxicity: A critical review of in vitro and in vivo dataFood and Cosmetics Toxicology, 1981