Immunological identification of four different polypeptides in ‘subunit VII’ of mammalian cytochrome c oxidase
- 9 July 1984
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 172 (2), 189-192
- https://doi.org/10.1016/0014-5793(84)81123-0
Abstract
Rat liver cytochrome c oxidase was separated by SDS-gel electrophoresis into 13 polypeptide bands. Monospecific antisera against the isolated polypeptides VIIa, VIIb and VIIc were raised in rabbits. Cytochrome c oxidase was blotted on nitrocellulose and incubated with the antisera. The antisera reacted only with their corresponding polypeptides, indicating no immunological relationship between polypeptides VIla, VIIb and VIIc. The data also exclude that these polypeptides are proteolytic breakdown products of larger subunits.Keywords
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