Pharmacological investigations of Punica granatum in glycerol-induced acute renal failure in rats
Open Access
- 1 January 2011
- journal article
- research article
- Published by Medknow in Indian Journal of Pharmacology
- Vol. 43 (5), 551-6
- https://doi.org/10.4103/0253-7613.84971
Abstract
Objective : The present study was designed to investigate the ameliorative potential and possible mechanism of hydroalcoholic extract of flowers of P. granatum in glycerol-induced acute renal failure (ARF) in rats. Materials and Methods : The rats were subjected to rhabdomyolytic ARF by single intramuscular injection of hypertonic glycerol (50% v/v; 8 ml/kg) and the animals were sacrificed after 24 hours of glycerol injection. The plasma creatinine, blood urea nitrogen, creatinine clearance, and histopathological studies were performed to assess the degree of renal injury. Results : Pretreatment with hydroalcoholic extract of flowers of P. granatum (125 and 250 mg/kg p.o. twice daily for 3 days) significantly attenuated hypertonic glycerol-induced renal dysfunction in a dose-dependent manner. BADGE (Bisphenol-A-diglycidyl ether) (30 mg/kg), a peroxisome proliferator-activated receptor (PPAR)-γ antagonist, and N(omega)-nitro-l-arginine-methyl ester (L-NAME) (10, 20, and 40 mg/kg), nitric oxide synthase inhibitor, were employed to explore the mechanism of renoprotective effects of Punica granatum. Administration of BADGE (30 mg/kg) and L-NAME (40 mg/kg) abolished the beneficial effects of P. granatum in glycerol-induced renal dysfunction. Conclusion : Hydroalcoholic extract of flowers of P. granatum has ameliorative potential in attenuating myoglobinuric renal failure and its renoprotective effects involve activation of PPAR-γ and nitric oxide-dependent signaling pathway.Keywords
This publication has 27 references indexed in Scilit:
- Peroxisome Proliferator-Activated Receptor (PPAR)γ Can Inhibit Chronic Renal Allograft DamageThe American Journal of Pathology, 2010
- Ameliorative potential of rosiglitazone in tibial and sural nerve transection-induced painful neuropathy in ratsPharmacological Research, 2009
- Pomegranate flower: a unique traditional antidiabetic medicine with dual PPAR‐α/‐γ activator propertiesDiabetes, Obesity and Metabolism, 2007
- Effects of a Pomegranate Fruit Extract rich in punicalagin on oxidation-sensitive genes and eNOS activity at sites of perturbed shear stress and atherogenesisCardiovascular Research, 2007
- Nitric oxide activation of peroxisome proliferator‐activated receptor gamma through a p38 MAPK signaling pathwayThe FASEB Journal, 2006
- Molsidomine, a nitric oxide donor and l-arginine protects against rhabdomyolysis-induced myoglobinuric acute renal failureBiochimica et Biophysica Acta (BBA) - General Subjects, 2005
- Anti-diabetic action of flower extract: Activation of PPAR-γ and identification of an active componentToxicology and Applied Pharmacology, 2005
- Role of glomerular nitric oxide in glycerol-induced acute renal failure.2000
- Biosynthesis and homeostatic roles of nitric oxide in the normal kidney.American Journal of Physiology-Renal Physiology, 1997
- Models of Glycerol-Induced Acute Renal Failure in RatsJournal of Basic and Clinical Physiology and Pharmacology, 1993