Dissecting the roles ofEscherichia colihydrogenases in biohydrogen production
Open Access
- 1 January 2008
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 278 (1), 48-55
- https://doi.org/10.1111/j.1574-6968.2007.00966.x
Abstract
Escherichia coli can perform at least two modes of anaerobic hydrogen metabolism and expresses at least two types of hydrogenase activity. Respiratory hydrogen oxidation is catalysed by two ‘uptake’ hydrogenase isoenzymes, hydrogenase -1 and -2 (Hyd-1 and -2), and fermentative hydrogen production is catalysed by Hyd-3. Harnessing and enhancing the metabolic capability of E. coli to perform anaerobic mixed-acid fermentation is therefore an attractive approach for bio-hydrogen production from sugars. In this work, the effects of genetic modification of the genes encoding the uptake hydrogenases, as well as the importance of preculture conditions, on hydrogen production and fermentation balance were examined. In suspensions of resting cells pregrown aerobically with formate, deletions in Hyd-3 abolished hydrogen production, whereas the deletion of both uptake hydrogenases improved hydrogen production by 37% over the parent strain. Under fermentative conditions, respiratory H2 uptake activity was absent in strains lacking Hyd-2. The effect of a deletion in hycA on H2 production was found to be dependent upon environmental conditions, but H2 uptake was not significantly affected by this mutation.Keywords
This publication has 23 references indexed in Scilit:
- The effect of nutrient limitation on hydrogen production by batch cultures of Escherichia coliInternational Journal of Hydrogen Energy, 2006
- Formate and its role in hydrogen production in Escherichia coliBiochemical Society Transactions, 2005
- Metabolically Engineered Rhodobacter sphaeroides RV strains for Improved Biohydrogen Photoproduction Combined with Disposal of Food WastesMarine Biotechnology, 2004
- Regulation of the Hydrogenase-4 Operon of Escherichia coli by the σ 54 -Dependent Transcriptional Activators FhlA and HyfRJournal of Bacteriology, 2002
- How bacteria get energy from hydrogen: a genetic analysis of periplasmic hydrogen oxidation in Escherichia coliInternational Journal of Hydrogen Energy, 2002
- Escherichia coli Strains Blocked in Tat-Dependent Protein Export Exhibit Pleiotropic Defects in the Cell EnvelopeJournal of Bacteriology, 2001
- A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-translocating formate hydrogenlyase systemMicrobiology, 1997
- Mutational analysis of the operon (hyc) determining hydrogenase 3 formation in Escherichia coliMolecular Microbiology, 1992
- Mechanism of regulation of the formate‐hydrogenlyase pathway by oxygen, nitrate, and pH: definition of the formate regulonMolecular Microbiology, 1991
- The fermentation pathways of Escherichia coliFEMS Microbiology Reviews, 1989