Kinetic and phenotypic changes in murine lymphocytes infected with murine gammaherpesvirus-68 in vitro
- 1 October 1999
- journal article
- Published by Microbiology Society in Journal of General Virology
- Vol. 80 (10), 2729-2736
- https://doi.org/10.1099/0022-1317-80-10-2729
Abstract
Primary infection with murine gammaherpesvirus-68 (MHV-68), as with other members of the gammaherpesvirus subfamily, is characterized by a lymphoproliferative phase. MHV-68 causes acute splenomegaly and an infectious mononucleosis-like syndrome in which there is expansion of the CD8+ T cell subset. In long-term infections, MHV-68 is associated with lymphoma development. In order to elucidate the mechanisms underlying the proliferative processes, the events following infection of murine splenocytes or purified murine B lymphocytes in vitro have been examined. MHV-68 infection prolonged the viability of murine splenocytes and stimulated cellular proliferation. Unlike Epstein–Barr virus and herpesvirus saimiri, MHV-68 did not cause growth transformation. Growth transformation did not occur even when cells with a predisposition to transformation were infected or when culture conditions were selected to enhance the viability of the cells. Following MHV-68 infection, the latency-associated viral tRNAs were transcribed. However, transcription of the other known latency- associated gene, M2, was not observed. In addition, there was no evidence of productive virus replication either by staining with antibodies specific for late virus antigens or by in situ hybridization for early and late mRNAs. In contrast to Epstein–Barr virus- and herpesvirus saimiri-infected lymphocytes, where episomal genomes are seen, Gardella gel analysis indicated that the primary lymphocytes infected by MHV-68 in vitro contained only linear virus DNA. This DNA was nuclease sensitive, indicating that, while MHV-68 was efficiently uncoated, its circularization in vitro was extremely inefficient. These results are discussed in terms of the host–virus interaction.Keywords
This publication has 25 references indexed in Scilit:
- 3 Growth Transformation of Human T CellsPublished by Elsevier ,1998
- Murine gammaherpesvirus 68 encodes tRNA-like sequences which are expressed during latency.Journal of General Virology, 1997
- The Kaposi sarcoma-associated herpesvirus (KSHV) is present as an intact latent genome in KS tissue but replicates in the peripheral blood mononuclear cells of KS patients.The Journal of Experimental Medicine, 1996
- Murine models of neoplasia: functional analysis of the tumour suppressor genesRb-1 andp53Cancer and Metastasis Reviews, 1995
- Thymocyte apoptosis induced by p53-dependent and independent pathwaysNature, 1993
- Requirement for a functional Rb-1 gene in murine developmentNature, 1992
- Stable growth transformation of human T lymphocytes by herpesvirus saimiri.Proceedings of the National Academy of Sciences, 1992
- Early events in Epstein-Barr virus infection of human B lymphocytesVirology, 1991
- Cloning and molecular characterization of the murine herpesvirus 68 genomeJournal of General Virology, 1990
- Progenitor and pre‐B lymphocytes transformed by epstein‐barr virusInternational Journal of Cancer, 1987