Morphological Components of Herpesvirus. III. Localization of Herpes Simplex Virus Type 1 Nucleocapsid Polypeptides by Immune Electron Microscopy
- 1 May 1981
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 54 (1), 39-46
- https://doi.org/10.1099/0022-1317-54-1-39
Abstract
Sera of individuals infected with adult T-cell leukaemia virus (ATLV) react predominantly with the polypeptides gp68, p24 and p19. These polypeptides were isolated from ATLV-infected MT-2 cells and virus. The radioiodinated polypeptides were used to quantify respective antibodies in individual ATLV carrier sera. Heteroantisera prepared in rabbits against isolated polypeptides facilitated studies on the biosynthesis of the core and envelope polypeptides of ATLV. Pulse-chase experiments revealed a polypeptide of mol. wt. 48000 (48K) as the precursor to the core polypeptides p24 and p19. A 28K polypeptide related to p19 appeared to be an early side-product of the gag gene or a translate of a defective viral message. Antiserum to the putative env gene product gp68 recognized gp68, gp66 and small amounts of gp62. In tunicamycin-treated cells gp68, gp66 and gp62 were no longer synthesized, but a 54K polypeptide reacted with antiserum to gp68. Polypeptide p54 is structurally related to gp68 and therefore apparently represents the unglycosylated form of gp68. Moreover, the apparent mol. wt. of p54 and p48 agree with those predicted for respective env and gag precursors from the nucleotide sequence of an ATLV provirus.This publication has 12 references indexed in Scilit:
- Identification of disulfide-linked protein complexes in the nucleocapsids of herpes simplex virus type 2Virology, 1979
- Isolation of a nucleocapsid polypeptide of herpes simplex virus types 1 and 2 possessing immunologically type-specific and cross-reactive determinantsJournal of Virology, 1979
- Direct evidence for 6-fold symmetry of the herpesvirus hexon capsomere.Proceedings of the National Academy of Sciences, 1978
- Structures of Polyoma Virus: on the Histone Component and Virion CoreJournal of General Virology, 1978
- Comparative Analyses of the Proteins and Antigens of Five HerpesvirusesJournal of General Virology, 1977
- Proteins of Epstein-Barr Virus. II. Electrophoretic analysis of the polypeptides of the nucleocapsid and the glucosamine- and polysaccharide-containing components of enveloped virusJournal of Virology, 1976
- Cell-free synthesis of equine herpesvirus type 3 nucleocapsid polypeptidesVirology, 1976
- The Replication of HerpesvirusesPublished by Springer Nature ,1974
- Some serological properties of herpes virus particles studies with the electron microscopeVirology, 1963
- The morphology of herpes virusVirology, 1960