A STIMULATION BY CYCLIC 3′,5′-ADENOSINE MONOPHOSPHATE OF AMINO ACID ACTIVATION AND POLYMERIZATION IN RETICULOCYTE HEMOLYSATES
- 1 November 1969
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 64 (3), 973-980
- https://doi.org/10.1073/pnas.64.3.973
Abstract
With reticulocyte supernatant, cyclic 3',5'-adenosine monophosphate at concentrations of 10(-3) to 10(-2)M causes stimulation of aminoacyl-tRNA synthetases for some, e.g., valine and leucine, but not all, amino acids; it is highest at nonsaturating concentrations of ATP. Similar concentrations of cyclic 3',5'-adenosine monophosphate are found to stimulate phenylalanine polymerization from phenylalanyl transfer ribonucleic acid on polyuridylic acid-charged reticulocyte ribosomes. The degree of stimulation is highest at low GTP concentrations. It is abolished by addition of phosphoenolpyruvate + pyruvate kinase, which stimulate similarly or more effectively at low GTP levels. Under the conditions of these experiments, cyclic 3',5'-adenosine monophosphate did not appreciably inhibit GTP hydrolysis.Keywords
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