On immunoglobulin heavy chain gene switching: two γ2b genes are rearranged via switch sequences in MPC-11 cells but only one is expressed
Open Access
- 1 January 1982
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (2), 611-630
- https://doi.org/10.1093/nar/10.2.611
Abstract
During B lymphocyte differentiation, switches in the expression of heavy chain immunoglobulin constant region (CH) genes occur by a novel DNA recombination mechanism. We have investigated the requirements of the CH gene switch by characterizing two rearranged γ2b genes from a γ2b producing mouse myeloma (MPC-11). One of the two γ2b genes is present in 2-3 copies per cell (γ2b strong hybridizer) while the other is present in ˜1 copy per cell (γ2b weak hybridizer). Genomic clones of the γ2b strongly hybridizing gene indicate that this is an abortive switch event between the Sγ3 and Sγ2b regions. How ever, clones of the γ2b weakly hybridizing gene suggest a functional rearrangement due to the presence of VH, JH and Sμ sequences. The switch-recombination sites of these rearranged γ2b genes and those of other CH genes show a high degree of preference for the sequence AGGTTG 5′ of either the Sμ donor site or the appropriate CH S acceptor site. AGGTTG and its analogs are rare in the Sμ region, are somewhat prevalent in Sα and in the case of Sμ are found 5′ of a tandemly repeated DNA sequence (GAGCT, GGGGT) comprising most of Sμ.Keywords
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