PHYSICAL PROPERTIES OF ANTIBODY TO BOVINE SERUM ALBUMIN AS DEMONSTRATED BY HEMAGGLUTINATION

Abstract

Rabbit antibovine serum albumin antisera were fractionated by zone electrophoresis on starch, zone ultracentrifugation in sucrose density gradients, and diethyl-aminoethyl-cellulose chromatography, and were assayed by the passive hemagglutination technique. Antisera had at least 2 antibodies one associated with a fraction chacterized as a gamma-2 globulin with a sedimentation rate of 7.3S and low anionic binding (fraction I), and one associated with a fraction characterized as a gamma-1 globulin (or beta-globulin) with a sedimentation rate of 20.4S and high anionic binding (fraction IV). The titer of fractions I and IV of early sera were approximately equal. On prolonged antigenic stimulation fraction IV agglutinin decreased in concentration and the relative concentration of fraction I agglutinin increased. Chromatographic analysis of hyperimmune sera yielded 2 additional hemagglutinating fractions with intermediate anionic binding. There was evidence that the hemagglutination method might measure antibody not measured by the quantitative precipitin technique.