IMAGE ANALYSIS OF CHROMATIN IN CELLS OF PREIMPLANTATION MOUSE EMBRYOS
Open Access
- 1 October 1974
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 63 (1), 227-233
- https://doi.org/10.1083/jcb.63.1.227
Abstract
Mouse two-celled embryos and blastulae were Feulgen stained and the DNA content of their nuclei was measured with an integrating microdensitometer. The cells considered on the basis of their nuclear DNA content to be in G(1), S, and G(2) phases of the cell cycle were selected and their total chromatin area and chromatin areas at different gray levels were measured by the image analyzing computer, Quantimet. The measurements were aimed at quantitation of several features of the chromatin morphology of cells in different functional states. The total area of chromatin was found to increase, and the mean density of chromatin to decrease, from the G(1) to the G(2) phase of the cell cycle in both two-celled embryos and blastulae. The area of chromatin decreased, and the mean density of chromatin increased, as embryos developed from two-celled to blastula stage. It was concluded that nuclear morphology in preimplantation mouse embryos depends on both the phase of the cell cycle and the stage of development. The method of image analysis described was found to be useful for quantitation of changes in chromatin morphology.Keywords
This publication has 18 references indexed in Scilit:
- Malignancy associated changes (MAC) in cells of buccal smears detected by means of objective image analysis.1974
- Re-evaluation of the Quantity of DNA in Cell Nucleus of Two Animal Species by Microspectrophotometry and AutoradiographyCYTOLOGIA, 1970
- Ultrastructural and autoradiographic studies of mouse cleavage stagesJournal of Anatomy, 1969
- An ultrastructural and cytological study of preimplantation development of the mouseJournal of Experimental Zoology, 1969
- Automated Analysis of Cellular Change in Histological SectionsScience, 1969
- FINE STRUCTURAL ALTERATIONS OF INTERPHASE NUCLEI OF LYMPHOCYTES STIMULATED TO GROWTH ACTIVITY IN VITROThe Journal of cell biology, 1968
- [Study of DNA synthesis during the period of embryo cleavage in mice (experiments in vitro)].1968
- Synthetic processes and early development in the Mammalian eggJournal of Experimental Zoology, 1964
- THE FINE STRUCTURE OF THE DNP COMPONENT OF THE NUCLEUSThe Journal of cell biology, 1963
- A cytochemical study of oogenesis and cleavage in the mouseJournal of Cellular and Comparative Physiology, 1950