An investigation of the intracellular fluid of calf embryo muscle

Abstract

The amino acids and peptides of the intracellular fluids of calf embryo muscle were examined by 2-dimensional chromatog-raphy on paper and compared with similar mixtures from muscle and serum of the adult animal. After removal of basic and neutral substances by chromatography on Amberlite IR4 the acidic components were fractionated by 1-dimensional paper chromatography. Sections corresponding to the spots observed on treatment with ninhydrin were eluted and the eluates were analyzed for P and further examined. Hydrolysis and repeated chromatography of these eluates indicated that aminoethylphoSphate and hydroxylysine in a form combined with P are present. The former substance was found to represent 2% and the latter 1% of the dialyzable P of the minced muscle.