In vivo transfer of plasmid pRAS1 between Aeromonas salmonicida and Aeromonas hydrophila in artificially infected Cyprinus carpio L.
- 9 February 2010
- journal article
- Published by Wiley in Journal of Fish Diseases
- Vol. 33 (3), 251-259
- https://doi.org/10.1111/j.1365-2761.2009.01118.x
Abstract
This study investigated the possible in vivo transfer of plasmid pRAS1 between Aeromonas salmonicida and A. hydrophila inhabiting two different organs of Cyprinus carpio L. To distinguish transconjugants from naturally occurring antibiotic resistant bacteria, twelve luminescent transposon-tagged A. hydrophila strains using miniTn5luxCDABEKm2 transposon were generated. In conjugal transfer experiments, fish were conditioned with the donor bacteria and subsequently immersed in water containing the recipient strain. Bacteria were recovered from gills and intestines and isolated by growth on selective plates. Transconjugants were identified by their resistance to the pRAS1 encoded antimicrobials and by light emission. In vivo transfer frequencies ranged between 10−3 and 10−6 and were somewhat lower in intestines, compared to gills. Transfer frequencies were also smaller relative to those obtained in vitro. The minimal amount of donor and recipient bacteria needed to yield detectable transconjugants in vivo was 1 × 104 CFU mL−1. Implications of this plasmid transfer in natural settings and its possible consequences to human health are discussed.Keywords
This publication has 45 references indexed in Scilit:
- Construction of a mini-Tn5-luxCDABE mutant library in Pseudomonas aeruginosa PAO1: A tool for identifying differentially regulated genesGenome Research, 2005
- Integron-Containing IncU R Plasmids pRAS1 and pAr-32 from the Fish Pathogen Aeromonas salmonicidaAntimicrobial Agents and Chemotherapy, 2003
- Sensitive In Situ Monitoring of a Recombinant Bioluminescent Yersinia enterocolitica Reporter Mutant in Real Time on Camembert CheeseApplied and Environmental Microbiology, 2002
- Routes of entry of Piscirickettsia salmonis in rainbow trout Oncorhynchus mykissDiseases of Aquatic Organisms, 1999
- Engineering theluxCDABEgenes fromPhotorhabdus luminescensto provide a bioluminescent reporter for constitutive and promoter probe plasmids and mini-Tn5constructsFEMS Microbiology Letters, 1998
- Invasive Infections Due to a Fish Pathogen,Streptococcus iniaeNew England Journal of Medicine, 1997
- High frequency transfer of a broad host range plasmid present in an atypical strain of the fish pathogen Aeromonas salmonicidaDiseases of Aquatic Organisms, 1996
- Amoxycillin resistance in Scottish isolates of Aeromonas salmonicidaJournal of Fish Diseases, 1994
- Characterization of Aeromonas salmonicida subsp. salmonicida: a comparative study of strains of different geographic originJournal of Applied Bacteriology, 1994
- Transfer and stability of drug resistance plasmids inEscherichia coli K12Microbial Ecology, 1984