URIDINE-SPECIFIC ANTIBODIES OBTAINED WITH SYNTHETIC ANTIGENS

Abstract

Completely synthetic antigens obtained by the chemical binding of uridine-5[image]-carboxylic acid to synthetic multichain polypeptides elicited, in rabbits antibodies with specificity toward uridine, as apparent from cross-precipitation and inhibition reactions. The attachment of the uridine derivative to a nonantigenic macromolecule converted it into an immunogen. The antibodies formed reacted with polyuridylic acid, heat-denatured RNA and DNA, but not with polyadenylic acid, native Eschericha coli RNA, or double-stranded calf thymus DNA.