Mutant lambda phage repressor with a specific defect in its positive control function.
- 1 April 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (7), 2236-2239
- https://doi.org/10.1073/pnas.79.7.2236
Abstract
The .lambda. phage repressor is both a positive and a negative regulator of gene transcription. A mutant .lambda. phage repressor that has specifically lost its activator function is described. The mutant binds to the .lambda. phage operator sites and represses the .lambda. phage promoters PR and PL. It fails to stimulate transcription from the promoter PRM. The mutation lies in that portion of repressors the amino-terminal domain, that mediates stimulation of PRM. The mutation apparently has altered that region of repressor which, in the wild-type, contacts RNA polymerase to activate transcription from PRM.This publication has 17 references indexed in Scilit:
- Congress versus cancerNature, 1981
- Improved methods for maximizing expression of a cloned gene: a bacterium that synthesizes rabbit β-globinCell, 1980
- Gene regulation at the right operator (OR) of bacteriophage λJournal of Molecular Biology, 1980
- Gene regulation at the right operator (OR) of bacteriophage λJournal of Molecular Biology, 1980
- Interactions between DNA-bound repressors govern regulation by the λ phage repressorProceedings of the National Academy of Sciences, 1979
- The lambda repressor contains two domains.Proceedings of the National Academy of Sciences, 1979
- DNA sequence of the bacteriophage λ cI geneNature, 1978
- Construction of plasmids carrying the cI gene of bacteriophage lambda.Proceedings of the National Academy of Sciences, 1976
- Lambda repressor turns off transcription of its own gene.Proceedings of the National Academy of Sciences, 1975
- Studies on lambda virulent mutantsMolecular Genetics and Genomics, 1973