Photoacoustic Imaging of Multiple Targets Using Gold Nanorods
- 20 August 2007
- journal article
- Published by Institute of Electrical and Electronics Engineers (IEEE) in IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
- Vol. 54 (8), 1642-1647
- https://doi.org/10.1109/tuffc.2007.435
Abstract
Photoacoustic (PA) imaging has been used mainly for anatomical and functional imaging. Although functionalized nanoparticles also have been developed for PA molecular imaging, only single targeting has been demonstrated. In this study, PA imaging of multiple targets using gold nanorods is demonstrated experimentally using HER2 and CXCR4 as target molecules. The two corresponding monoclonal antibodies were conjugated to two types of gold nanorod with different aspect ratios. Gold nanorods with mean aspect ratios of 5.9 and 3.7 exhibited peak optical absorptions at 1000 and 785 nm, respectively. Appropriate selection of laser irradiation wavelength enhances PA signals by 7-12 dB and allows signals from gold nanorods corresponding to specific bindings to be distinguished. This approach potentially allows the expression levels of different oncogenes of cancer cells to be revealed simultaneously.Keywords
This publication has 17 references indexed in Scilit:
- Photoacoustic flow measurements by use of laser-induced shape transitions of gold nanorodsOptics Letters, 2005
- AMD3465, a monomacrocyclic CXCR4 antagonist and potent HIV entry inhibitorBiochemical Pharmacology, 2005
- In VitroCharacterization of a Bivalent Anti-HER-2 Affibody with Potential for Radionuclide-Based DiagnosticsCancer Biotherapy & Radiopharmaceuticals, 2005
- Photoacoustic measurement of epidermal melaninPublished by SPIE-Intl Soc Optical Eng ,2003
- Bioconjugated gold nanoparticles as a contrast agent for detection of small tumorsPublished by SPIE-Intl Soc Optical Eng ,2003
- Scanometric DNA Array Detection with Nanoparticle ProbesScience, 2000
- Self-assembly of nanoparticles into structured spherical and network aggregatesNature, 2000
- Spectral Properties and Relaxation Dynamics of Surface Plasmon Electronic Oscillations in Gold and Silver Nanodots and NanorodsThe Journal of Physical Chemistry B, 1999
- Organization of 'nanocrystal molecules' using DNANature, 1996
- A DNA-based method for rationally assembling nanoparticles into macroscopic materialsNature, 1996