Measurement of the pH of Frozen Buffer Solutions by Using pH Indicators
- 1 January 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 81 (1), 163-168
- https://doi.org/10.1093/oxfordjournals.jbchem.a131431
Abstract
A method was established to estimate the pH change of several buffer solutions on freezing by using a combination of pH indicators. Among more than 30 buffer solutions examined, almost half exhibited a pH change in the temperature range between freezing point and 220°K; the results were tabulated. Glycerol was found to suppress the pH changes because of its “salt buffer” effect.Keywords
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