Sandwich Enzymoimmunoassay of Hepatitis B Surface Antigen (HBsAg)
- 1 October 1978
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 35 (4), 219-223
- https://doi.org/10.1111/j.1423-0410.1978.tb02925.x
Abstract
A sandwich enzymoimmunoassay (EIA) procedure was developed for the detection of HBsAg using Fab'' of rabbit anti-HBsAg conjugated with .beta.-D-galactosidase from Escherichia coli with anti-HBsA-coated silicone rubber discs as a solid phase. EIA could detect 1 ng/ml of HBsAg. It was as sensitive as radioimmunoassay (RIA, AusRia II) and about 30-fold more sensitive than reversed passive hemagglutination assay (RPHA, ReverseCell). EIA and RIA could detect more HBsAg-positive sera than RPHA.This publication has 16 references indexed in Scilit:
- Solid-phase enzyme immunoassay for hepatitis B surface antigen.Clinical Chemistry, 1977
- Enzyme-immunoassay in the detection of hepatitis B surface antigenJournal of Immunological Methods, 1977
- Enzyme-linked sandwich immunoassay of macromolecular antigens using the rabbit antibody-loaded silicone piece as a solid phaseFEBS Letters, 1976
- Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen.Journal of Clinical Pathology, 1976
- Coupling Fab′ fragment of rabbit anti‐human IgG antibody to β‐d‐galactosidase and a highly sensitive immunoassay of human IgGFEBS Letters, 1975
- Separation and Purification of Hepatitis-associated Antigen into Morphologic Types by Zonal UltracentrifugationThe Journal of Infectious Diseases, 1972
- Hemagglutination Assay for Antigen and Antibody Associated with Viral HepatitisScience, 1970
- Dissociation of Rabbit γ-Globulin into Half-Molecules after Reduction of One Labile Disulfide Bond*Biochemistry, 1964
- A Simple Chromatographic Method for Preparation of Gamma Globulin.Experimental Biology and Medicine, 1960
- The serum proteins in multiple myelomatosisBiochemical Journal, 1940