Colorimetric Determination of Microgram Quantities of Urea
- 1 February 1970
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 3 (2), 79-87
- https://doi.org/10.1080/00032717008067782
Abstract
A sensitive colorimetric method of determining urea is described. It involves measurement of the absorbance of the red color formed when urea is heated with diacetyl monoxime and thiosemicarbazide under acidic conditions (phosphoric acid-sulfuric acid medium). The method is accurate and precise, and it permits determination of microgram amounts of urea in the presence of ammonium, nitrate, amino acids, amides, and amino sugars.Keywords
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