The use of polarography in the examination of ketosteroids related to cholesterol
- 1 December 1955
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 61 (4), 681-688
- https://doi.org/10.1042/bj0610681
Abstract
A standard type of recording polarograph was modified and improved polarograms were obtained. A buffer with an ethanol content of 90% (v/v) facilitated polarography of C19-, and C21- and C27-ketosteroids in the free state and as the betainyl hydrazones. Some ketonic bile acids were also investigated. A method for the examination of C27-ketosteroids in biological material was developed. The separation of cholestan-3-one and coprostan-3-one as 2:4-dinitrophenylhydrazones is described.Keywords
This publication has 4 references indexed in Scilit:
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