An improved technique for the cultivation of Plasmodium falciparum in vitro without daily medium change
- 1 January 1985
- journal article
- research article
- Published by Taylor & Francis in Pathogens and Global Health
- Vol. 79 (4), 379-384
- https://doi.org/10.1080/00034983.1985.11811935
Abstract
A simple method is described allowing growth of Plasmodium falciparum in suspension cultures with 1% outdated human erythrocytes without the need for medium change for three to four days. Depending on the initial parasitaemia, final parasitaemias of from 10% to 30% can be obtained. Growth is exponential and asynchronous with a mean multiplication rate of 7·7±1·0 (n=5) -fold per 48-hour cycle. The method is suitable for cultivation of a wide range of laboratory strains of P. falciparum, as well as for primary isolations of clinical material.This publication has 6 references indexed in Scilit:
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