The metabolism of dl-1-deoxyl-1-fluoroglycerol

Abstract

DL-1-Deoxy-l-fluoroglycerol is toxic to the rat, mouse and guinea pig. Death is associated with convulsions, bradycardia, lowering of body temperature and accumulation of citrate, particularly in heart and kidney. DL-1-fluoroglycerol did not affect respiration or citrate level in homogenates or slices of kidney, brain, liver or muscle, nor did it increase the citrate level in the perfused heart. Functional hepatectomy made the rat insensitive to DL-l-deoxy-1-fluoroglycerol. Perfused rat liver converted DL-1-deoxy-l-fluoroglycerol into a compound (or compounds) capable of causing citrate accumulation in a perfused heart or in kidney particles. Chromatography on silica gel of the perfusate from a heart-liver preparation indicated that fluoroacetate was the principal citrate-accumulating factor.