Effect of Salvianolic Acid A, a Depside from Roots ofSalvia miltiorrhiza, on Gastric H+, K+-ATPase

Abstract
Salvianolic acid A, a depside from the roots of Salvia miltiorrhiza, inhibited pig gastric H+,K+-ATPase and pNPPase with 50% inhibition values (IC50) of 5.2 × 10-7 M and 1.7 × 10-6 M, respectively. Kinetic studies revealed that the inhibition patterns induced by Salvianolic acid A were competitive with respect to ATP and noncompetitive with respect to K+. Salvianolic acid A (25 mg/kg, i.p.) significantly inhibited acid secretion in pylorus-ligated rats. At the same dose it also showed a significant reduction in the formation of gastric lesion induced by water immersion and restraint stress. These results suggest that Salvianolic acid A shows antisecretory and antiulcer activity by inhibiting the gastric H+,K+-ATPase.